Qrt Pcr Data Analysis. There are two ways to analyze qpcr data. Pfaffl 2001 a new mathematical model for relative quantification in real time rt pcr article.
Ct pcr cycle a typical qpcr run has around 40 cycles. Double delta ct analysis and the relative standard curve method. The higher the ct 30 35 the less the mrna detected is present because you need more cycles of amplification to detect the fluorescence.
Real time quantitative reverse transcription pcr is a major development of pcr technology that enables reliable detection and measurement of products generated during each cycle of pcr process.
Qrt pcr analyzing your data further notes for consideration and questions for discussion. The cdna is then used as the template for the qpcr reaction. In this method rna is first transcribed into complementary dna cdna by reverse transcriptase from total rna or messenger rna mrna. This technique became possible after introduction of an oligonucleotide probe which was designed to hybridize within the target sequence.
