Positive Indirect Elisa Test. The sandwich elisa test detects a protein or antigen by capturing it between two antibodies. This test must be used if the lyme igg igm antibody serology or lyme igg iga igm ifa is positive or equivocal.
This color is directly proportional to the amount of bound sample antibody. Unknowns that generate a signal that is stronger than the known sample are positive and those that generate weaker signal are negative. Hence indirect elisa is more sensitive than direct elisa.
For example an elisa test for viral rna can detect it a positive test not detect it a negative test or be indeterminate borderline test.
This color is directly proportional to the amount of bound sample antibody. One known antibody one unknown antibody and an unknown antigen. The addition of an enzyme substrate chromogenic reagent causes color to develop. In an elisa four types of false positive reactions can be encountered regardless of the antigens coated on the elisa plate.
