Indirect Elisa Test Procedure. Place five 17 100 mm test tubes in a rack and add 3 ml blocking buffer to the last four tubes. Prepare developing reagent dilutions antibody alkaline phosphatase conjugates.
Then a solution containing non reacting proteins. Place five 17 100 mm test tubes in a rack and add 3 ml blocking buffer to the last four tubes. Direct indirect sandwich or competitive.
Procedure of indirect elisa coat the micro titer plate wells with antigen.
Prepare developing reagent dilutions antibody alkaline phosphatase conjugates. Direct elisa test uses only primary antibodies to detect the antigen while indirect elisa uses both primary and secondary antibodies. Enzyme linked immunosorbent assay elisa test is the most widely used type of immunoassay. Elisa is a rapid test used for detecting or quantifying antibody ab against viruses bacteria and other materials or antigen ag.
