Indirect Elisa Test Diagram. The assay uses a solid phase type of enzyme immunoassay eia to detect the presence of a ligand commonly a protein in a liquid sample using antibodies directed against the protein to be measured. The enzyme linked immunosorbent assay elisa ɪ ˈ l aɪ z ə ˌ iː ˈ l aɪ z ə is a commonly used analytical biochemistry assay first described by engvall and perlmann in 1971.
It is used as a diagnostic tool to find out whether the patient has been exposed to a particular type of virus or another infectious agent antigen and whether the body has produced antibodies against the infection. Permeabilized and blocked target proteins are detected with antibodies. I discovered that an elisa uses antibodies that bind to their respective target antigens on an elisa plate and it includes antibodies that are linked to an enzyme that converts a substrate into a colored product when the target antigen is present.
As it turns out her name is an acronym for enzyme linked immunosorbent assay or elisa.
The assay uses a solid phase type of enzyme immunoassay eia to detect the presence of a ligand commonly a protein in a liquid sample using antibodies directed against the protein to be measured. Next an enzyme labeled secondary antibody is bound to the first antibody. First an unlabeled primary antibody which is specific for the antigen is applied. It is used as a diagnostic tool to find out whether the patient has been exposed to a particular type of virus or another infectious agent antigen and whether the body has produced antibodies against the infection.
