Indirect Elisa Procedure. Dilute the antigen to a final concentration of 20 µg ml in pbs or other carbonate buffer. Indirect elisa detects antibodies from the test sample.
Indirect elisa protocol buffers and reagents. Wrap coated plates in plastic wrap to seal and incubate for 2 hr at 370c in an incubator. Samples with antibodies are added and washed.
Direct elisa test uses only primary antibodies to detect the antigen while indirect elisa uses both primary and secondary antibodies.
See direct elisa protocol buffers and reagents. Wrap coated plates in plastic wrap to seal and incubate for 2 hr at 370c in an incubator. Steps process of indirect elisa micro well plates are incubated with antigens washed up and blocked with bsa. Used for the detection of serum antibodies against human immune deficiency virus hiv hiv.
