Indirect Elisa Diagram. We can use indirect elisa to detect antibodies against many types of pathogens including borrelia burgdorferi lyme disease and hiv. In an indirect elisa we quantify antigen specific antibody rather than antigen.
Different strategies for both capture and detection are used in elisa. The assay uses a solid phase type of enzyme immunoassay eia to detect the presence of a ligand commonly a protein in a liquid sample using antibodies directed against the protein to be measured. References 1 the enzyme linked immunosorbent assay elisa bulletin of the world health organization.
Elisa is an antigen antibody reaction.
This is the difference between direct and indirect elisa. In 1971 elisa was introduced by peter perlmann and eva engvall at stockholm university in sweden. References 1 the enzyme linked immunosorbent assay elisa bulletin of the world health organization. National library of medicine 1976.