Direct And Indirect Elisa Test. There are many permutations of this assay including an indirect elisa described here but i narrowed it down to two options. Direct enzyme linked immunosorbent assay or the elisa is the method to detect and measure the antigen concentration the sample contains.
First what kind of elisa did i want to run. It can be performed via two processes known as direct or indirect elisa. The main difference between direct and indirect elisa is that in direct elisa the primary antibody is directly conjugated to the detection enzyme whereas in indirect elisa a secondary antibody which is complementary to the primary antibody is conjugated with the detection enzyme.
Direct elisa test uses only primary antibodies to detect the antigen while indirect elisa uses both primary and secondary antibodies.
For most assay systems test solutions containing 1 to 100 ng ml of antigen can be accurately measured. The addition of an enzyme substrate chromogenic reagent causes color to develop. Does direct or indirect elisa test have a secondary antibody. Elisa assay this immunological assay lecture explains about the elisa test procedure and principle behind the elisa assay including direct indirect and sa.
